Mouse Trypsinogen Activation Peptide (TAP) ELISA Kit

Cat.#:

JL11967

Regulatory Status:

RUO

Alternative:

TAP

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

15.6-1000pg/mL

Sensitivity:

6.2pg/mL

Specificity:

This kit recognizes Mouse TAP in samples. No significant cross-reactivity or interference between Mouse TAP and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Stem Cells

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TAP

Trypsinogen activation peptide (TAP) is the amino-terminus peptide released by the activation of trypsinogen. The inappropriate activation of trypsinogen within the pancreas results in the release of TAP into the blood, urine, and peritoneum. The concentration of urinary TAP is thought to correlate directly with the severity of acute pancreatitis, reflecting the degree of trypsinogen activation in the pancreas.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse TAP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Mouse TAP and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110